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Promoter Ligation

  • Put together ligation reactions between the J23100 promoter and both the pSB1K3 and J61002 plasmid backbones. Promoter solutions used were the working solution of 10um, 50x diluted working solution, and 500x diluted working solution. Each was combined with an equal volume of plasmid solution in a ligation mix. The purpose of this procedure was to add a promoter part, surrounded by biobricks, to each plasmid backbone so that target genes can be inserted behind the promoter.