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Plasmid Backbone Isolation
The three wells to the left of the ladder contained pSB1K3 samples, while the two wells to the left of the ladder contained J61002 samples. Two pSB1K3 samples and one J61002 sample showed DNA. The bands near the 1000bp marker (furthest downward on the gel) contain the plasmid inserts that were digested out. The bands between 2000 and 3000bp contained plasmid backbones and were separated from adjacent bands (undigested plasmid) and excised from the gel.