|Cloning of Atrolysin A from Crotalus atrox||Main project page|
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DNA Extraction and PCR
Starting on the left. Lanes are: A1, E1, A2, E2, A3, Ladder, E3, Negative control, Positive control. The "A" samples contain 5 microliters of DNA and the "E" samples contain 0.5 microliters of DNA. The "1" samples were placed at 65 degrees C in the PCR, the "2" samples were placed at 55 degrees C, and the "3" samples and the negative and positive controls were placed at 50 degrees C. There were no bands present which means that the amplification did not work.