840:153g:Projects/project26/2012/08/30

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Cloning of Atrolysin A from Crotalus atrox Main project page
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Locating Shed

  • After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone, it is the atrolysin A toxin. We were unable to find a DNA sequence but found an mRNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.