Team Members: Sanju Timilsina and Parul Sirohi
Project title: Over expression of Acetyl CoA carboxylase biotin carboxylase (accC) gene, the subunit of Acetyl CoA carboxylase(ACC) enzyme in E.coli to enhance fatty acid accumulation for Biofuel production"
Source organism E coli culture:
- 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on)
- Flask was then labelled 1 and 2, LB, date and group number .
- 25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
- Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours and we decided to take our sample out of the shaker before our next day class.