From OpenWetWare
Jump to: navigation, search

9/27 - Today we performed RE digest and agarose gel electrophoresis using the DNA that we extracted and purified last week with the corrected protocol. Analysis of the gel once again shows no or insufficient DNA extraction. We will next try to extract DNA using the specialized albeit more complex procedure developed specifically for citrus DNA extraction.

We also diluted our 4 custom primers, which had arrived last week, with PCR-ready (UV-irradiated) distilled water to make 100 µM solutions.