840:153g:Projects/project18/2011/10/27

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Cloning of catA gene of Acinetobacter calcoaceticus strain ADP1 to observe its expression in E.coli. As we could not see any amplified copy of our bacterial gene catA of Acinetobater calcoaceticus when we run the agarose gel and there was no proper result of positive control as we used catA gene primers instead of Psb1A7 primers. We repeated PCR of AC 2 sample today. The primers which we used fr AC2 did not have prefix and suffix. The positive control was Psb1A7 and we used its primers today and run the PCR using gas18 protocol and the annealing temperatures were 44c-60c. The PCR was set for overnight and will be stopped tomorrow. The samples will be kept in 4C until tuesday. We will do Agarose gel elctrophoresis on tuesday to check whether the desired gene is amplified.