840:153g:Projects/project18/2011/10/18

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Cloning of catA gene of Acinetobacter calcoaceticus strain ADP1 to observe its expression in E.coli. Today we proceeded with our Bacterial DNA isolation method. In the previous class we did till addition of Isopropanol and further steps were proceeded and Bacterial DNA was isolated. 100ul of DNA in two were isolated. Then the sample from two tubes were taken and Restriction digestion was done with EcoRI and PstI. To observe wether the isolated bacterial DNA can be cut with restriction enzymes. We run Agarose gel with digested and undigested samples to observe the DNA of Interest. After the gel was run for 30 mins we observed it under UV light and we could find our DNA which is 936 bp and we could even observe that our bacterial DNA is digestible.