From OpenWetWare
Jump to: navigation, search

Cloning of catA gene of Acinetobacter calcoaceticus strain ADP1 to observe its expression in E.coli. The rehydration culture of Acinetobacter calcoaceticus was plated in BHI medium with different concentrations sample 10ul, 50ul, 100ul, 300ul. The plates were incubated in incubator at 37c for overnight and growth of colonies were observed next day. On 12th october There were big and tiny colonies grown in the plates. We count the colonies and we took Two big colonies from the plate containing 100ul of sample were taken and inoculated in 4ml of BHI broth and kept it incubator for overnight at 37c without shaking. The plates were stored in 4c.

On 13th october, we observed the growth of bacteria as the medium was turbid though we put it in incubator shaker. The whole sample were taken in two different tubes and Bacterial DNA isolation Method was applied to isolate its DNA. The protocol was taken from group 20. We followed till isopropanol step and left it on the bench. Further steps will be proceeded on October 18th.