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Cloning of gene of Acinetobacter calcoaceticus strain ADP1 to observe its expression in E.coli. Date:0ct/04/2011

Today we transform Plasmid DNA of our promoter and the sample (PSB1A7#3 and #4)on freshly prepared LB media Plates.We took 10 LB plates in which 9 were with Ampicillin(AMP) and 1 was with no AMP.After then we prepared Brain Heart Infusion(BHI)Agar and Broth.We prepared 4 BHI agar plates and stored it in freeze for further use and we stored the broth in freeze too.And we recovered our bacterial sample and kept it in incubator to let it grow and get activated.