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Cloning of catA gene of Acinetobacter calcoaceticus strain ADP1 to observe its expression in E.coli.

The transformed plates were observed today.The growth of competent cells were observed in antibiotic media and antibiotic free media.So we are not sure about wether the ampicillin is not working or the competent cells have developed resistance for the ampicillin. Today we took 5 plates:- a) Lb media plate without antibiotic b) kanamycin plate and other other three were ampicillin plates which were prepared at different dates.

All the plates were inoculated with 20ml of competent cells,to check wether the cells have developed the resistance for antibiotics.Then the plates were kept in the incubator for over-night and results will be obeserved tomorrow.

By:Sahil Sharma