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Tuesday September 20, 2011 We prepared LB media and autoclaved it. While waiting for the media to autoclave we extracted our promoter part(Bba_K118011)from Biobrick Spring 2011 Kit 2 and stored it in tube at –80 degree for using it in next lab day.As the color of sample was red we were able to figure out that we successfully extracted the part from the kit.After autoclave was done we added Ampicillin to the media poured it into plates and stored the plate's for further use. By Sindhu Adhikari.