Today we repeated the enzyme digest protocol from 9/27 increasing the total volume of mix to 36 microliters and ran 30 microliters rather than 15 on an electrophoresis gel. Undiluted DNA was also run and the volume was increased as well. Our promoter parts arrived from Registry of Biological parts. Amp R plants were being made so someone from our group will come in on Monday 10/3/11 and do a streak plate for all three of the transformed bacteria that arrived (with promoter parts). Still waiting on gel to finish, If DNA is confirmed present we will begin a PCR reaction next class. If DNA is not present we will repeat DNA isolation on a much larger quanitity of isolate from our plant specimen.