840:153g:Projects/project16/2011/11/29
From OpenWetWare
Jump to navigationJump to search
Project name | Main project page Previous entry |
Transforming ligations into competent cellsWe transformed our plasmids into competent bacteria and plated them. We had 27 plates, 26 were LB ampicillin plates, 1 was non-ampicillin plate. Of the 26 LB ampicillin plates, 24 of them contained our our combinations of wintergreen and RFP genes. The other 2 LB ampicillin plates each contained controls, 1 had our positive control (competent plasmid), the other had our negative control (just RFP). The non-ampicillin plate was a non-selective control which is just competent cells.
|