In today's lab we attempted another transformation of competant E. Coli cells with our ligated plasmids made during lab last thursday. After transformation, the E.Coli were spread onto agar plates containing ampicilin, and left to incubate overnight. Megan will return to the lab tomorrow afternoon to check colony growth. If there are colonies present (preferably white colonies over blue colonies) these colonies will be innoculated in LB media and left to grow in the shaker until thursdays lab. We do expect more growth than our previous attempt because we used a higher concentration of our DNA to ligate into the plasmids.
Written by Kim Lovik