In today's lab we transformed competent Ecoli using our plasmids that had been ligated with our gene during the previous lab. We set up 6 experimental colonies, using our 6 different DNA samples, along with 2 control plasmids. A negative control group was also mixed using no plasmid. These Ecoli samples were incubated at 37 degrees celcius for one hour and then plated onto ampicilin agar plates. They will sit overnight until tomorrow at 4pm when Megan will check cultures for growth. She will select colonies (if present) and innoculate them in LB media with ampicilin, which will be used for plasmid isolation during Thursdays lab.
Written by Kim Lovik