In today's lab, we took our T-vector samples from Tuesday's lab and transformed them into competent E. coli cells using the pGEM-T Easy Vector System protocol. We then took the agar plates we had prepared on Tuesday, spread the samples on each plate, and placed them in incubation. Tomorrow we will take the plates out of incubation, wrap them in parafilm, and store them. Axel will isolate colonies to be grown until next lab. We will make a plan for our next step after we see the results of the plates next lab. If we see growth, we will extract the DNA from the plates and send the samples to sequencing in order to find out if we isolated the correct gene, our OOMT2 gene.
Written by Megan Hughes