In today's lab we took the gene fragments that were extracted from the gel last Thursday and transformed them into T-Vectors. Since each sample appeared to contain a different concentration of the gene, we first calculated the amount of each gene solution to used, based on it's brightness compared to the ladder (known concentration 20ng/ul). We then mixed each individual solution (6 samples, 2 control samples) and placed them in the refrigerator to be used for E.Coli transformation during the next lab period. We also used time during this lab to prepare and pour agar plates to be used to grow E.Coli for the second part of our experiment.
Written by Kim Lovik