3 February 2009

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Date: 3 February 2009; Time:0800; Agenda: Biofilm formation at 21deg and 30deg for 48hr and 72hr.

  • 5ml of overnight culture added to 150 ml LB broth in flask for 6.5hr in a 37deg incubator,200rpm.

Biofilm formation:

  • Similar procedures carried out to grow biofilm, only that now they are left to grow for 48hr and 72hr at 21 deg and 30deg. 50rpm.
  • Columns 1-6: M9 culture; Columns 7-12: LB culture