27 December 2008

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Date: 27 December 2008; Time: 0930; Agenda: Biofilm formation at 1 temperature for 48hr and 72hr.

  • 5ml of overnight culture added to 150 ml LB broth in flask for 5hr incubation in a 37deg incubator.

Biofilm formation:

  • Similar procedures carried out to grow biofilm, only that now they are left to grow for 48hr and 72hr at 30deg. 30rpm.
  • Columns 1-6: M9 culture(centrifuged --> We decided to continue using this method as the M9 inoculum did not have any colonies growing when plated! (refer to 26 December)); Columns 7-12: LB culture
  • Similar plating done to verify McFarland standards.