| MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
NOTES
|
|
|
|
Tue Feb 7
|
ANS
|
JRR
|
Orientation
|
* Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
|
|
|
| 1
|
1
|
Thur Feb 9
|
JN
|
JRR
|
Amplify aptamer-encoding DNA
|
* M1D1 Lecture
* M1D1 Pre-Lab
* Writing tutor talk
|
| 1
|
2
|
Tue Feb 14
|
JN
|
JRR
|
Purify aptamer-encoding DNA
|
* M1D2 Lecture
* M1D2 Pre-Lab
* T/R WAC talk
* W/F WAC talk
|
| 1
|
3
|
Thur Feb 16
|
JN
|
JRR
|
Prepare RNA by IVT
|
* M1D3 Lecture
* M1D3 Pre-Lab
* Oral presentation talk
|
|
|
|
President's Day Feb 20
|
No lab Feb 21 or Feb 22
|
| 1
|
4
|
Thur Feb 23
|
JN
|
JRR
|
Purify RNA and run affinity column
|
* M1D4 Lecture
* M1D4 Pre-Lab
|
| 1
|
5
|
Tue Feb 28
|
JN
|
JRR
|
RNA to DNA by RT-PCR
|
* M1D5 Lecture
* M1D5 Pre-Lab
|
| 1
|
6
|
Thur Mar 1
|
JN
|
JRR
|
Post-selection IVT and journal club
|
* M1D6 Lecture
* M1D6 Pre-Lab
|
| 1
|
7
|
Tue Mar 6
|
JN
|
JRR
|
Aptamer binding assay
|
* M1D7 Lecture
* M1D7 Pre-Lab
|
| 1
|
8
|
Thur Mar 8
|
JN
|
JRR
|
Journal club
|
* M1D8 Lecture
|
|
|
| 3
|
1
|
Tue Mar 13
|
ANS
|
HHH
|
Start-up biomaterials engineering
|
* M3D1 Lecture
* M3D1 Pre-Lab
Module 1 Draft Due
|
|
|
| 2
|
1
|
Thur Mar 15
|
AJ
|
FF
|
Start-up protein engineering
|
* M2D1 Lecture
* M2D1 Pre-Lab
Module 1 Computational Assignment Due
|
| 2
|
2
|
Tue Mar 20
|
AJ
|
FF
|
Site-directed mutagenesis
|
* M2D2 Lecture
* M2D2 Pre-Lab
|
| 2
|
3
|
Thur Mar 22
|
AJ
|
FF
|
Bacterial amplification of DNA
|
* M2D3 Lecture
* M2D3 Pre-Lab
|
|
|
|
Spring Break Mar 26-30
|
No lab all week
|
| 2
|
4
|
Tue Apr 3
|
AJ
|
FF
|
Prepare expression system
|
* M2D4 Lecture
* M2D4 Pre-Lab
|
| 2
|
5
|
Thur Apr 5
|
AJ
|
FF
|
Induce protein and evaluate DNA
|
* M2D5 Lecture
* M2D5 Pre-Lab
Module 1 Final Report Due
|
| 2
|
6
|
Tue Apr 10
|
AJ
|
FF
|
Characterize protein expression
|
* M2D6 Lecture (got through slide 15)
* M2D6 Pre-Lab
|
| 2
|
7
|
Thur Apr 12
|
AJ
|
FF
|
Assess protein function
|
* M2D7 Lecture
* M2D7 Pre-Lab
|
|
|
|
Patriot's Day April 16-17
|
No lab Apr 17 or Apr 18
|
|
|
| 3
|
2
|
Thur Apr 19
|
ANS
|
HHH
|
Initiate cell culture
|
* M3D2 Lecture
|
|
|
|
Tue Apr 24
|
ANS
|
HHH
|
|
No lab or lecture. Module 2 Draft due MONDAY April 23rd by 1 pm. (Changed to April 24th.)
|
| 3
|
3
|
Thur Apr 26
|
ANS
|
HHH
|
Testing cell viability
|
* M3D3 Lecture
|
| 3
|
4
|
Tue May 1
|
ANS
|
HHH
|
Preparing cells for analysis
|
* M3D4 Lecture
* M3D4 Pre-Lab
|
| 3
|
5
|
Thur May 3
|
ANS
|
HHH
|
Initiating transcript and protein assays
|
* M3D5 Lecture
* M3D5 Pre-Lab
|
| 3
|
6
|
Tue May 8
|
ANS
|
HHH
|
Transcript-level analysis
|
Module 2 Final Report Due
* M3D6 Lecture
* M3D6 Pre-Lab
|
| 3
|
7
|
Thur May 10
|
ANS
|
HHH
|
Protein-level and wrap-up analysis
|
Module 3 Summary Due
|
| 3
|
8
|
Tue May 15
|
ANS
|
HHH
|
Student presentations
|
|
|
|
|
|
|
Thur May 17
|
|
|
No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
|
|
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