20.109(F09):System engineering research article guidelines

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Day 1

In advance of Module:

  • streak out NB334 onto LB+Amp+Cam+Kan
  • streak out NB188 onto LB+Amp+Kan
  • streak out NB5 on LB+Amp

In advance of Day 1:

  • Prepare 50 ml bottles of indicator media but don't add H2O
  • Grow ONs of NB5 in LB+Amp

On lab day:

  • In AM subculture NB5, 1 ml/20ml LB+Amp on RT shaker. Induce with IPTG at start of lab period.