10X BPTE electrophoresis buffer
Used as the electrophoresis buffer during denaturing (via glyoxal) agarose gel electrophoresis of RNA.
- 100 mM PIPES
- 300 mM Bis-Tris
- 10 mM EDTA
The final pH of this 10x buffer is ~6.5.
- Prepare by adding the following to 90 ml of distilled H2O
- 3 g of PIPES (free acid)
- 6 g of Bis-Tris (free base)
- 2 ml of 0.5 M EDTA
- Treat the solution with final concentration of 0.1% DEPC for 1 hour at 37°C