| Reagent
| 1.5x
| 2.5x
| 3.5x
| 6.5x
| 9.5x
| 12.5x
|
| Fwd. Primer 10 μM
| 1.5 μL
| 2.5 μL
| 3.5 μL
| 6.5 μL
| 9.5 μL
| 12.5 μL
|
| Rev. Primer 10 μM
| 1.5 μL
| 2.5 μL
| 3.5 μL
| 6.5 μL
| 9.5 μL
| 12.5 μL
|
| 10 mM ea dNTPs
| 1.5 μL
| 2.5 μL
| 3.5 μL
| 6.5 μL
| 9.5 μL
| 12.5 μL
|
| DNA Template
| 5 μL of Lyse'n Go per reaction; Lyse'n Go thermocycle takes ~18 min. Can start Lyse'n Go cycle before making master mix
|
| 10x Rxn Buffer (Invitrogen (-MgCl2))
| 7.5 μL
| 12.5 μL
| 17.75 μL
| 32.5 μL
| 47.5 μL
| 62.5 μL
|
| 50 mM MgCl2
| 2.25 μL
| 3.75 μL
| 5.25 μL
| 9.75 μL
| 14.25 μL
| 18.75 μL
|
| milliQ H2O
| 45.75 μL
| 76.25 μL
| 106.75 μL
| 198.25 μL
| 289.75 μL
| 381.25 μL
|
| Homemade Taq
| 7.5 μL
| 12.5 μL
| 17.5 μL
| 32.5 μL
| 47.5 μL
| 62.5 μL
|
| Add 45 μL of reaction master mix to each Lyse'n Go reaction
|
