Yu:IPP

Solution recipe for 500mL IPP150.

1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker	25mM Tris-HCl
2. Add 15mL of 5M NaCl	150mM NaCl
3. Add 5mL of 10% NP-40	0.1% NP-40
4. Bring volume to 500mL with millipore H2O in a graduated cylinder	Volume to 500mL

Solution recipe for 500mL IPP300.

1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker	25mM Tris-HCl
2. Add 30mL of 5M NaCl	300mM NaCl
3. Add 5mL of 10% NP-40	0.1% NP-40
4. Bring volume to 500mL with millipore H2O in a graduated cylinder	Volume to 500mL