Wikiomics:BLAST tutorial (exercises)

From OpenWetWare
Jump to navigationJump to search
  • blastp with shuffled sequence: note that all hits must be wrong!
>Shuffled_protein
MNALFNGRYVRQNLRFAGFARVDRERPPLTWFQVSAIPLFARGFAGAIDSSRISRSGEAF
VGCVCLAQPCFVGAADGEADLRAGVGSVTVAVNGSLLLFALG
  • what is this nucleotide sequence?
>nucleotide_1
ATATAGTTTTTAATTAGGAGGATTTTATCAATGAAAATATCAATACTATACAGTAGCAAGACAGGAAAAA
CCGAAAGAGTTGCCAAGTTAATTGAAGAAGGTGTGAAGAGGTCTGGAAATATTGAAGTAAAAACTATGAA
TTTAGATGCAGTTGATAAAAAGTTCTTACAAGAATCAGAAGGAATTATTTTTGGAACACCAACTTATTAT
  • same sequence mutated:
>nucleotide_2
ATATAGTATTTAATTAGGAGGATTTCATCAATGACACTCTCAATACTATACAGTAGCAAG
ACAGGGAAAACAGAAAGAGTTGCCAAGGTAATTGAGGAAGGTGTGAAGAGGTCTCAAAAT
AATGAAGTAAGAACTGTGAATCTAGATGCAGTTCATAATAAGTTCTTCGAAGAATCAGGA
GGCATTCTTTTTGGAACACCAACTTATGAT
  • what is it? Any remote similarity?
>protein_1
HIGSDTDGDDDSETEGEWNLKEGGSIDAELSHSRKAKKVESLLRKLHTKDILPEEVSS
GSHLHTRSPMVLFLSLAVPTDKVVMFYKALFTEAELKAPLESLKRFQNNGDARTGKSA
IFMLGGGHFAGAVISHEPVKVKGQLLNEELPYLQKIQSINVLESKTFHRYTTRRKQGG
  • play with your favorite gene/protein