Van Oudenaarden Lab:W02C12.3
Progress:
Probes designed: (03-04-2010 - Christoph Engert)
Probes coupled:
Probes purified:
Probes tested:
Dyes available:
>W02C12.3a (spliced + UTR - 1558) cagcaattca tacacaattg atcgtccccg tccatataac cgctgaaaaa aaggaaaaaa aATGGCCGAT GACGACGACC GCGTTGACAT TTCCAACGAG ACGCTTGCCT CTTTGGTGGG TCGACCGATG AGCTCGTCGG GCAATCGGAT TCGAGCCACC CCATCCAGAC GGCGGCTGGT TTCTGCGCCG ACGAACCCGA GGTGGTCACC ATCCGCAGAC GATCGGCGTC TTTTTTCATA TGTCTCGTCG ATGCGGTCGG ATAGTAACAG TAGCAGCAAG TCACCATCTC CACCGTCGGC CAAAATGTCT AAAAGGCCGA ATGGCCCGTA CTCTGTTAGA AAATATCAGC CCCGCGGATC CCCACCCAGT GACGGCAGCG ACAAAATTCA CAGATTCGGA GAGAGCCCAA CGCCCGGTGG TGTCGGTGGT GTATTTGGCA CAGAGCTTGA CGACCTGATC ATTGATGAGC TCATGGGCAT GGAAGATGAT CAGCGAATGC GTCCAGGAGC GACACGACCG ATGACGATTG GTGGAGAAAA AACCATGTCG ATGGCAAGAC CGATTCCAGG CGCCAGCTCC CGGGCTGGCT CAGGACACTC TGGAAGCCCG ATCACAATTC CAAACGCAAT GTCGAACAAT TTCAGACAGG TCGTCTCAAG TTCGGCGCCG ACGAGTTCGA TCGACATTGA GAAGATGATT GGCGCCGTGT CGAACGGCGG TGGGAATAGT GGCGGTGATA ATGATCCGGA GGACTATTAC CGCGACCGCA GGAAGAAGGA CATTCACAAT ATGATTGAAC GCCGACGAAG ATATAATATA AATGACAGGA TTAAGGAGCT CGGACAAATG TTGCCGAAGA ACACATCAGA AGACATGAAA CTCAACAAAG GAACAATTCT AAAAGCCTCG TGTGACTACA TCCGAGTGCT CCAAAAAGAT CGCGAGCAAG CGATGAAAAC CCAACAGCAG CAGAAATCGC TGGAATCGAC GGCTCACAAG TACGCCGACC GGGTGAAAGA GCTCGAGGAG ATGCTCGCCA GGCAGGGAGT GCAGGTCCCG CCATCGCATC TTCCACCGAT CCCGAAAGTG ATCGAGCGAC CGATTAAGCA GGAAATCGAC GAGTCGCCAC CAAATCACAC GCCGACCGGA TCGTTTGTGT CCTCATCCGG CTTCCTATCC GAGGTGACAA ACAACACCGC CGCCATGCAG ATCACATCGC CGAACGACTC GCGCCCGAAC AACTTCATGA ACAACTCGGC GCCGTCGGAC AGCTTCTTCT CGGTCGGATC GGCTAGCCCG CCAGATTATA GGACGAGCAG CGGCACCGCG TCGTGGAAGC TGCCCGGATC GAACGCCTTC TCCGACCTCA TGATGGACGA TCTCAACCCA ATGATGAACG GCGATCCACT CATCTCATCG GCCGGCGCTC ATCCATCACC ACACTTCCAC AGCTCACAAA TGTCACCAGA CATTCATTGG GACGCCTCTG GCTTCTCCCC AGACCCGATC AACACCCAAC AGTCAAACTC GGGTCATTAT CACATGGACT TTTCGTAA
Name of probe set: hlh-30 Probes designed on Wed, 03 Mar 10 23:37:47 -0700 42 probes designed for target of length 1497
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
tctcgttggaaatgtcaacg, hlh-30_1, 1, 21, 45 aaaaagacgccgatcgtctg, hlh-30_2, 2, 155, 50 accgcatcgacgagacatat, hlh-30_3, 3, 177, 50 gacttgctgctactgttact, hlh-30_4, 4, 202, 45 tttagacattttggccgacg, hlh-30_5, 5, 233, 45 ttttctaacagagtacgggc, hlh-30_6, 6, 263, 45 tctgtgaattttgtcgctgc, hlh-30_7, 7, 314, 45 aaatacaccaccgacaccac, hlh-30_8, 8, 356, 50 atcaggtcgtcaagctctgt, hlh-30_9, 9, 379, 50 catgcccatgagctcatcaa, hlh-30_10, 10, 401, 50 gacgcattcgctgatcatct, hlh-30_11, 11, 423, 50 tttctccaccaatcgtcatc, hlh-30_12, 12, 459, 45 atcggtcttgccatcgacat, hlh-30_13, 13, 484, 50 attgtgatcgggcttccaga, hlh-30_14, 14, 538, 50 attgttcgacattgcgtttg, hlh-30_15, 15, 560, 40 aacttgagacgacctgtctg, hlh-30_16, 16, 582, 50 tcaatgtcgatcgaactcgt, hlh-30_17, 17, 610, 45 attatcaccgccactattcc, hlh-30_18, 18, 662, 45 tgtgaatgtccttcttcctg, hlh-30_19, 19, 708, 45 tatcttcgtcggcgttcaat, hlh-30_20, 20, 733, 45 gagctccttaatcctgtcat, hlh-30_21, 21, 761, 45 gtgttcttcggcaacatttg, hlh-30_22, 22, 784, 45 gttgagtttcatgtcttctg, hlh-30_23, 23, 806, 40 gaggcttttagaattgttcc, hlh-30_24, 24, 829, 40 ttttggagcactcggatgta, hlh-30_25, 25, 856, 45 tttcatcgcttgctcgcgat, hlh-30_26, 26, 878, 50 agcgatttctgctgctgttg, hlh-30_27, 27, 901, 50 gtacttgtgagccgtcgatt, hlh-30_28, 28, 923, 50 atctcctcgagctctttcac, hlh-30_29, 29, 952, 50 tttcgggatcggtggaagat, hlh-30_30, 30, 1007, 50 atttcctgcttaatcggtcg, hlh-30_31, 31, 1036, 45 atgaggacacaaacgatccg, hlh-30_32, 32, 1086, 50 tttgtcacctcggataggaa, hlh-30_33, 33, 1111, 45 ttcggcgatgtgatctgcat, hlh-30_34, 34, 1144, 50 tgttcatgaagttgttcggg, hlh-30_35, 35, 1173, 45 tcctataatctggcgggcta, hlh-30_36, 36, 1233, 50 tccatcatgaggtcggagaa, hlh-30_37, 37, 1297, 50 cgttcatcattgggttgaga, hlh-30_38, 38, 1320, 45 tttgtgagctgtggaagtgt, hlh-30_39, 39, 1380, 45 tcccaatgaatgtctggtga, hlh-30_40, 40, 1402, 45 ttgactgttgggtgttgatc, hlh-30_41, 41, 1446, 45 tccatgtgataatgacccga, hlh-30_42, 42, 1468, 45
Input sequence with probe locations:
atggccgatgacgacgaccgcgttgacatttccaacgagacgcttgcctctttggtgggtcgaccgatga
gcaactgtaaaggttgctct
Probe # 1, 45% GC
gctcgtcgggcaatcggattcgagccaccccatccagacggcggctggtttctgcgccgacgaacccgag
gtggtcaccatccgcagacgatcggcgtcttttttcatatgtctcgtcgatgcggtcggatagtaacagt
gtctgctagccgcagaaaaa tatacagagcagctacgcca tcattgtca
Probe # 2, 50% GC Probe # 3, 50% GC Probe # 4
agcagcaagtcaccatctccaccgtcggccaaaatgtctaaaaggccgaatggcccgtactctgttagaa tcgtcgttcag gcagccggttttacagattt cgggcatgagacaatctt , 45% GC Probe # 5, 45% GC Probe # 6, 45% GC
aatatcagccccgcggatccccacccagtgacggcagcgacaaaattcacagattcggagagagcccaac tt cgtcgctgttttaagtgtct
Probe # 7, 45% GC
gcccggtggtgtcggtggtgtatttggcacagagcttgacgacctgatcattgatgagctcatgggcatg
caccacagccaccacataaa tgtctcgaactgctggacta aactactcgagtacccgtac
Probe # 8, 50% GC Probe # 9, 50% GC Probe # 10, 50% GC
gaagatgatcagcgaatgcgtccaggagcgacacgaccgatgacgattggtggagaaaaaaccatgtcga
tctactagtcgcttacgcag ctactgctaaccacctcttt tacagct Probe # 11, 50% GC Probe # 12, 45% GC Probe #
tggcaagaccgattccaggcgccagctcccgggctggctcaggacactctggaagcccgatcacaattcc accgttctggcta agaccttcgggctagtgtta g
13, 50% GC Probe # 14, 50% GC P
aaacgcaatgtcgaacaatttcagacaggtcgtctcaagttcggcgccgacgagttcgatcgacattgag tttgcgttacagcttgtta gtctgtccagcagagttcaa tgctcaagctagctgtaact robe # 15, 40% GC Probe # 16, 50% GC Probe # 17, 45% GC
aagatgattggcgccgtgtcgaacggcggtgggaatagtggcggtgataatgatccggaggactattacc
ccttatcaccgccactatta
Probe # 18, 45% GC
gcgaccgcaggaagaaggacattcacaatatgattgaacgccgacgaagatataatataaatgacaggat
gtccttcttcctgtaagtgt taacttgcggctgcttctat tactgtccta
Probe # 19, 45% GC Probe # 20, 45% GC Probe # 21
taaggagctcggacaaatgttgccgaagaacacatcagaagacatgaaactcaacaaaggaacaattcta attcctcgag gtttacaacggcttcttgtg gtcttctgtactttgagttg ccttgttaagat , 45% GC Probe # 22, 45% GC Probe # 23, 40% GC Probe # 24,
aaagcctcgtgtgactacatccgagtgctccaaaaagatcgcgagcaagcgatgaaaacccaacagcagc tttcggag atgtaggctcacgaggtttt tagcgctcgttcgctacttt gttgtcgtcg 40% GC Probe # 25, 45% GC Probe # 26, 50% GC Probe # 27
agaaatcgctggaatcgacggctcacaagtacgccgaccgggtgaaagagctcgaggagatgctcgccag tctttagcga ttagctgccgagtgttcatg cactttctcgagctcctcta , 50% GC Probe # 28, 50% GC Probe # 29, 50% GC
gcagggagtgcaggtcccgccatcgcatcttccaccgatcccgaaagtgatcgagcgaccgattaagcag
tagaaggtggctagggcttt gctggctaattcgtc
Probe # 30, 50% GC Probe # 31, 45%
gaaatcgacgagtcgccaccaaatcacacgccgaccggatcgtttgtgtcctcatccggcttcctatccg cttta gcctagcaaacacaggagta aaggataggc
GC Probe # 32, 50% GC Probe # 33
aggtgacaaacaacaccgccgccatgcagatcacatcgccgaacgactcgcgcccgaacaacttcatgaa tccactgttt tacgtctagtgtagcggctt gggcttgttgaagtactt , 45% GC Probe # 34, 50% GC Probe # 35, 45% GC
caactcggcgccgtcggacagcttcttctcggtcggatcggctagcccgccagattataggacgagcagc gt atcgggcggtctaatatcct
Probe # 36, 50% GC
ggcaccgcgtcgtggaagctgcccggatcgaacgccttctccgacctcatgatggacgatctcaacccaa
aagaggctggagtactacct agagttgggtt
Probe # 37, 50% GC Probe # 38,
tgatgaacggcgatccactcatctcatcggccggcgctcatccatcaccacacttccacagctcacaaat actacttgc tgtgaaggtgtcgagtgttt
45% GC Probe # 39, 45% GC
gtcaccagacattcattgggacgcctctggcttctccccagacccgatcaacacccaacagtcaaactcg
agtggtctgtaagtaaccct ctagttgtgggttgtcagtt agc Probe # 40, 45% GC Probe # 41, 45% GC Pro
ggtcattatcacatggacttttcgtaa ccagtaatagtgtacct be # 42, 45% GC
