User talk:Nkuldell/SAGA swap

Notes on SGF73 swap

H.s. to S.c. swap of SGF73

at [[1]] fill in paper details here...
and for Dom's paper [2]
File:Macintosh HD-Users-nkuldell-Desktop-HelmlingerHMG04 1.pdf

alignment SGF73 S. pombe vs S. cerevisiae

protein alignment using blastp at [3]
SGF73 protein in S. cerevisiae is 657 aa
SPCC126.04c protein in S. pombe is 344 aa

Notes from discussion with Dom:

• • middle block of alignment has non-canonical Zn finger that is conserved with human SGF73 and that he showed by mass spec can bind Zn. This block is exquisitely conserved across species.
  • first block of alignment has homology to SGF11 as well, including a more traditional Zn finger.

codon usage SGF73 S. pombe vs S. cerevisiae

Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [4]

alignment SGF29 S. pombe vs S. cerevisiae

protein alignment using blastp at [5]
SGF29 protein in S. cerevisiae is 259aa
SPBC1921.07c protein in S. pombe is 244 aa

codon usage SGF29 S. pombe vs S. cerevisiae

Codon Usage Comparison using GeneArt tool [6]