User talk:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/31

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Steve Koch 00:27, 1 November 2009 (EDT): It is amazing to me that D2O has been used to stabilize vaccines very effectively. (see my earliest two articles tagged here: http://delicious.com/skoch3/d2o) Since kinesin is so unstable, I can only imagine that storing it in heavy water would make it much more stable (perhaps already shown by your assay working >24 hours). We gotta try that!

Steve Koch 00:49, 1 November 2009 (EDT): Also, it would be really cool to do motility with "heavy" kinesin as well (if the heads are isotopically heavier, they'd diffuse slightly slower, probably changing motility...although now I'm learning that it may change chemistry as well...)

Steve Koch 00:54, 1 November 2009 (EDT): Also, heavy water ice cubes sink in water (and I bet they're delicious too). I gotta try that sometime. Heavy water liposomes would sink in water (if they're bigg-ish, right?), and light water liposomes would float in heavy water. You could figure out the identity of a liposome based on its density. I don't know why I think that's very cool. You should write down all of those ideas we had yesterday, otherwise, I'm going to keep posting these silly thoughts :) (Maybe it'd be a way of studying diffusion of water through liposomes; or a way of "delivering" heavy water to a localized spot (briefly; by bursting liposomes in the vicinity))

Steve Koch 01:34, 1 November 2009 (EDT): I think "kinetic solvent isotope effect" may be the relevant terminology for us

Steve Koch 16:04, 1 November 2009 (EST): wikipedia article on "kinetic isotope effect" does not mention solvent (KSIE) at all, and it's all about chemistry, nothing about modulation of binding interactions due to solvent difference.

Steve Koch 23:49, 1 November 2009 (EST): article about classic article on measuring pD with regular pH meter

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