What was done today
Today we made Au-NPs with the protein's invertase and BSA
Procedure
New Nanoparticle Synthesis:
Please choose two from the following sets!
- Bovine serum albumin
- Ratios
- 105
- 110
- 115
- 120
- 125
- 130
- 135
- 140
- 145
- 150
- Catalase
- Ratios
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
- Invertase
- Ratios
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
- Elastin
- Ratios
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
Notes
Table 1. Preparation of Gold Standards
Concentration(μg/mL)
|
Volume of stock Au solution(mL)
|
Volume Deionized water added(mL)
|
10 |
0.1 |
9.9
|
20 |
0.2 |
9.8
|
30 |
0.3 |
9.7
|
40 |
0.4 |
9.6
|
50 |
0.5 |
9.5
|
Table 2. Preparation of BSA stock solution
Preparation of BSA stock solution
|
'
|
Molecular weight (g/mol) |
66000
|
mass (g) |
0.0432
|
volume stock (L) |
0.03
|
Concentration (M) |
2.18182E-05
|
Table 3. Preparation of Invertase Stock solution
Preparation Invertase Stock
|
'
|
Molecular weight (g/mol) |
135000
|
mass invertase (g) |
0.1015
|
volume stock (L) |
0.025
|
concentration (M) |
3.00741E-05
|
Table 4. Preparation of BSA-AuNP solutions
Ratio
|
Amount of gold added(mL)
|
concentration BSA (M)
|
volume BSA (mL)
|
volume Water added (mL)
|
105 |
0.347660664 |
2.38095E-06 |
0.545634921 |
4.106704416
|
110 |
0.347660664 |
2.27273E-06 |
0.520833333 |
4.131506003
|
115 |
0.347660664 |
2.17391E-06 |
0.498188406 |
4.15415093
|
120 |
0.347660664 |
2.08333E-06 |
0.477430556 |
4.174908781
|
125 |
0.347660664 |
0.000002 |
0.458333333 |
4.194006003
|
130 |
0.347660664 |
1.92308E-06 |
0.440705128 |
4.211634208
|
135 |
0.347660664 |
1.85185E-06 |
0.424382716 |
4.22795662
|
140 |
0.347660664 |
1.78571E-06 |
0.40922619 |
4.243113146
|
145 |
0.347660664 |
1.72414E-06 |
0.395114943 |
4.257224394
|
150 |
0.347660664 |
1.66667E-06 |
0.381944444 |
4.270394892
|
Table 5. Preparation of Invertase-AuNP solutions
Ratio
|
Amount gold added (mL)
|
Concentration Invertase (M)
|
Volume Invertase (mL)
|
Volume water added (mL)
|
30 |
0.347660664 |
8.33333E-06 |
1.38546798 |
3.266871356
|
50 |
0.347660664 |
0.000005 |
0.831280788 |
3.821058548
|
70 |
0.347660664 |
3.57143E-06 |
0.593771992 |
4.058567345
|
90 |
0.347660664 |
2.77778E-06 |
0.46182266 |
4.190516676
|
110 |
0.347660664 |
2.27273E-06 |
0.377854904 |
4.274484432
|
130 |
0.347660664 |
1.92308E-06 |
0.31972338 |
4.332615956
|
150 |
0.347660664 |
1.66667E-06 |
0.277093596 |
4.37524574
|
170 |
0.347660664 |
1.47059E-06 |
0.244494349 |
4.407844987
|
190 |
0.347660664 |
1.31579E-06 |
0.218758102 |
4.433581234
|
210 |
0.347660664 |
1.19048E-06 |
0.197923997 |
4.454415339
|
|