User:Wesley J. Houston/Notebook/MAP/2013/04/04

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4/4/13 The liquid cultures did not grow. Prepped two more liquid cultures from the ligation plate, as well as performed another ligation. > Ligation   1 2 Insert DNA (KAH182) 7.0 --- Vector DNA (KAH201) 1.7 1.7 2x lgn buf (Roche) 9.7 9.7 T4 ligase (NEB) 1.0 1.0 dH2O 0.6 7.6   20 μL 20 μL Follow quick transformation protocol Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar Ligation Plate results (lig : neg crtl)04/01/13: Ligation 1. KAH201 (S/P)/3403, 50 ng + KAH182 (X/P)/1675, 50 ng 0 2. KAH201 (S/P)/3403, 50 ng 0 The ligation was unsuccessful.