User:Tk/Notebook/MF-xfm/2008/04/14

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Transformations Some small colonies of a few of the older plates likely junk PCR of transposons 20 ul reactions 0.6 ul ME0 primer cycle 95/5 min, (95/20s, 55/20s, 72/3.0 min) x 32 wells: 83 ng/ul cut PCR'd transposon transposome cells from culture B of initial transformations (streaked plate for growth test) Setup of cultures for tomorrow 50 ml cultures infected with 5 ml 0.5 ml 50 ul 5 ul