User:Tara K. Luckau/Notebook/Team ConGen/2012/01/09

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Contents


CNHY Multiplex

Gel

  • run this gel slow and long to hopefully be able to distinguish approximate allele range for each marker (using pooled DNA of CNHY)
Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 120 V
27 µL GelRed 6 µL ladder 1.25 hour


  • good banding; I'm assuming smearier bands have greater allelic range (as opposed to mispriming, as corroborated with corresponding gradient PCR)
  • Allelic range, by marker:
-- Acos3: 200 bp
-- Acos5: 160 bp
-- Ai5013: 220 bp
-- Ai5033: 260 bp
-- Ai5035: 200-280 bp
-- Ai5037: 150-300 bp
-- Ai5043: 300-350 bp
-- Ai5057: 240 bp
-- Ai5062: 180-300 bp
-- Ai5071: 275 bp




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