User:Tara K. Luckau/Notebook/Team ConGen/2011/06/27

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Contents


Fragment Analysis Submission

  • Submit samples from 23 June 2011 PCR2 (condition indicated by below)
  • Submit samples from 24 June 2011 ( below)


  • Frag Submission Map:


  • FedEx Tracking# 875047691856


  • UAGC Submission# KXBJ

Evaporation test for Shipping

  • used 22 June 2011 PCR (failed)
  • sealed with flat-top 8-well strip caps, left on Team ConGen computer 23 June - 27 June


  • Plate Weight
- Before (23 June) After (27 June)
Trial 1 19.44g 19.22g
Trial 2 19.41g 19.24g
Trial 3 19.36g 19.26g
Average 19.40g 19.24g


Conclusion

  • 0.16 g loss of liquid via evaporation after 4 days at room temperature
  •  0.16 g \bullet \tfrac{1 mL}{1 g} \bullet \tfrac{1000 \mu L}{1 mL} = 160 \mu L
  • 32 rxn * 10µL - 40µL gel = 280µL sample in the plate; so >1/2 liquid is lost!
  • BUT: there's still at least ~ 3µL left in all wells, which is plenty for fragment analysis
  • BUT: when shipping, plate will be cold, and will only take 2 days
  • thus, sealing this way is a-okay!




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