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Fragment Analysis Submission
- Submit samples from 23 June 2011 PCR2 (condition indicated by
below)
- Submit samples from 24 June 2011 (
below)
-
-
- FedEx Tracking# 875047691856
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Evaporation test for Shipping
- used 22 June 2011 PCR (failed)
- sealed with flat-top 8-well strip caps, left on Team ConGen computer 23 June - 27 June
| - | Before (23 June) | After (27 June)
|
| Trial 1 | 19.44g | 19.22g
|
| Trial 2 | 19.41g | 19.24g
|
| Trial 3 | 19.36g | 19.26g
|
| Average | 19.40g | 19.24g
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Conclusion
- 0.16 g loss of liquid via evaporation after 4 days at room temperature
-
- 32 rxn * 10µL - 40µL gel = 280µL sample in the plate; so >1/2 liquid is lost!
- BUT: there's still at least ~ 3µL left in all wells, which is plenty for fragment analysis
- BUT: when shipping, plate will be cold, and will only take 2 days
- thus, sealing this way is a-okay!
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