|
Fragment Analysis Submission
- Submit samples from 23 June 2011 PCR2 (condition indicated by
 below)
- Submit samples from 24 June 2011 (
 below)
- FedEx Tracking# 875047691856
Evaporation test for Shipping
- used 22 June 2011 PCR (failed)
- sealed with flat-top 8-well strip caps, left on Team ConGen computer 23 June - 27 June
| - |
Before (23 June) |
After (27 June)
|
| Trial 1 |
19.44g |
19.22g
|
| Trial 2 |
19.41g |
19.24g
|
| Trial 3 |
19.36g |
19.26g
|
| Average |
19.40g |
19.24g
|
Conclusion
- 0.16 g loss of liquid via evaporation after 4 days at room temperature
- [math]\displaystyle{ 0.16 g \bullet \tfrac{1 mL}{1 g} \bullet \tfrac{1000 \mu L}{1 mL} = 160 \mu L }[/math]
- 32 rxn * 10µL - 40µL gel = 280µL sample in the plate; so >1/2 liquid is lost!
- BUT: there's still at least ~ 3µL left in all wells, which is plenty for fragment analysis
- BUT: when shipping, plate will be cold, and will only take 2 days
- thus, sealing this way is a-okay!
|
Tara's Lab Notebook
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