Oct 7 - LCMS-2010 Introduction
Sample prep
200ppm solutions of the three different SHMP products
- Ash - 0.02497g
- Yanbu - 0.02275g
- AP - 0.01992g
All diluted to 100mL with DI water
LCMS-2010 Notes
order of operation
- LCMS solution software
- Turn on gas 120 psi Nitrogen
- Open Window
- LCMS solution Analysis 1>Data>Instrument parameters >Scan mode 50-600>Sample Settings> 5 min run time because there was not a column
- Must always make sure controller has a stop at end of program
- Created a Tami Tinsley folder in CHEM 605, save all data there
- Tested SHMP for Ash for Test 1 > 1ml per min, isocratic flow
- 093015Tunning program
- 5µL column use 10µL of sample to inject > must use blunt tip for injection
- Test 2 reduced flow rate to 0.250 for both pump A and B
Results
- Test 1 images, pump flow 0.500 mL/min
- Test 2 images, pump flow 0.250 mL/min
- Higher pump flow produced better resolution
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