User:Steven J. Koch/Notebook/Kochlab/2009/09/15/EarI pBR322

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Steve Koch 20:29, 15 September 2009 (EDT): Using restriction cross-correlate software & pBR322 sequence from genbank (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/pBR322.gbk.txt), I find two EarI sites:

EarI sites on pBR322
  • 2350
  • 4154

Thus, should produce two fragments, one approx 2556; the other approx 1804 bp. From the top strand, the overhangs read:

  • 2350
    • GCT (This is the overhang we have on our adapter duplex)
  • 4154
    • TTT

If we gel extract the big piece (approx 2556), it will be complementary to a 5' GCT overhang. So, I think we are all good. This is consistent I think with my earlier work (see below).

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