User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/10/05

Drosophila melanogaster Genetics	Main project page Previous entry      Next entry
Spermatogenesis Dissect 3 sets of testis 8 from Tdt-Myb 2 x 8 from GFP-Myb, H2Av-RFP Fix 30' in 4% Formaldehyde at RT Wash 4 x 5' at RT Block 1 hr at RT in 2% NGS Primary Antibody Tdt-Myb with 1:1000 anti-mRFP O/N at 4°C GFP-Myb with 1:5 anti-BAM O/N at 4°C GFP-Myb with 1:100 anti-SA O/N at 4°C