User:Raquel Lara/Notebook/481-2018/2018/09/26

Biomaterials Design Lab

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Objective set up dual pump try to form beads Description set-up the same as 9/21/18: Used syringe to join peek tubing Determine the rate of the glutaraldehyde needed to have a 2:1 ratio with PVA Determine the length of Tygon tubing at which glutaraldehyde and PVA mix for at least 5 minutes Data Pump set-up New rates: Red pump: 0.01 mL/min Grey pump: 0.02 mL/min Recalculated Length: V= pi*r^2*l 0.01 mL/min * 5 min) = 3.14 * 0.00158 cm^2 * l 0.05 mL / (3.14 * 0.00158) = l l = 10.078 cm Notes Previous calculation was off by a factor 10 so we increased the length to 101 cm instead for this second trial. Epoxy used to connect PEEK tubing and Tygon to prevent leaking. Polymer beads formed in ethyl acetate