User:Phillip R. Smith

From OpenWetWare

Jump to: navigation, search

Contents

Contact Info

Phillip R. Smith
Phillip R. Smith

Research papers by Phillip Smith View Phil Smith's profile on LinkedIn

Education

  • 2005-Present, PhD Candidate, Chemical Engineering, Stanford University
  • 2005, MS, Chemical Engineering, Brigham Young University
  • 2003, BS, Chemical Engineering, Brigham Young University

Papers

Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.02.048

Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.03.172

Technical Presentations

Phillip Smith | GCEP Symposium 2011 from Cyperus Media.com on Vimeo.

Research interests

  1. Protein Biotechnology (molecular engineering and directed protein evolution)
  2. Biochemical Reaction Engineering (bioprocess development)
  3. Synthetic Enzyme Pathways
  4. Protein Purification
  5. High-throughput Biotechnology

Biotechnology skillset

  • Protein overexpression (aerobic and anaerobic fermentation, shake-flask to 10L scale)
  • Protein purification (ion-exchange, affinity, and other chromatographic methods using an AKTA purifier and home-made systems)
  • Directed enzyme evolution
  • Protein engineering -- site directed mutagenesis, fusion protein design and construction
  • PCR, gene synthesis, mutagenesis
  • Spectrophotometric assay development

Useful links

Worth checking out

Personal tools