User:Phillip R. Smith
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Contact Info
- Phillip R. Smith
- Stanford University
- Member of the Swartz Lab, in the Department of Chemical Engineering
- Keck Science Building, Stanford, CA
- Email me through OpenWetWare
Education
- 2005-Present, PhD Candidate, Chemical Engineering, Stanford University
- 2005, MS, Chemical Engineering, Brigham Young University
- 2003, BS, Chemical Engineering, Brigham Young University
Papers
Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.02.048
Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.03.172
Technical Presentations
Phillip Smith | GCEP Symposium 2011 from Cyperus Media.com on Vimeo.
Research interests
- Protein Biotechnology (molecular engineering and directed protein evolution)
- Biochemical Reaction Engineering (bioprocess development)
- Synthetic Enzyme Pathways
- Protein Purification
- High-throughput Biotechnology
Biotechnology skillset
- Protein overexpression (aerobic and anaerobic fermentation, shake-flask to 10L scale)
- Protein purification (ion-exchange, affinity, and other chromatographic methods using an AKTA purifier and home-made systems)
- Directed enzyme evolution
- Protein engineering -- site directed mutagenesis, fusion protein design and construction
- PCR, gene synthesis, mutagenesis
- Spectrophotometric assay development
Useful links
- My Resume
- E._coli_genotypes
- PCR_Overlap_Extension
- Reverse Complement
- Reverse Translate
- DNAworks
- DNA<->RNA->Protein
- dsDNA MW Calculator
- DNA MW Calculator
- Protein MW Calculator
- Protein Composition Analyzer
- Buffer Calculator
- Robetta
- ascii codes
- Sequetech
- Primer Design Basics
- Gel Densitometry Using Image J
