User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/09/05
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Parts assembly-PCRsABSTRACT
I made two reactions to amplify PFROR2 sequence and another two to amplify HydG sequence. The temperatures and times of the cycles were: Initial denaturation: 98°C for 30s
-Denaturation: 98°C for 10s -Annealing: 60°C for 30s -Extension: 72°C for 1 min Final extension: 72°C for 5 min Hold 4°C
The temperatures and times of the cycles were: Initial denaturation: 98°C for 30s
-Denaturation: 98°C for 10s -Annealing: A gradient of temperature from 55°C to 62°C -Extension: 72°C for 1 min Final extension: 72°C for 7 min Hold 4°C
PCR gel was run at 120 Volts for 60 minutes 1μL of dye was used 2μL of PCR product were loaded 2μL of ladder were loaded
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