User:Paula Satisteban Vela
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- Nury Paula Santisteban Vela
- Memorial University of Newfoundland
- St. John's, NL, Canada
- nps071@mun.ca
Education
- 2019 Ph.D., Memorial University of Newfoundland, NL, Canada
Thesis title: Interaction of Antimicrobial Peptides with the Gram Positive and Gram Negative Cell Envelope
Supervisor: Valerie Booth. Co-Supervisor: Michael Morrow
- During my time as a Ph.D. student, I developed and tested protocols for growing deuterium-rich bacteria to be able to perform deuterium NMR measurements in whole cells. I also tested the changes in the 2H NMR spectra of E. coli and B. subtilis in the presence of Antimicrobial peptides. Finally, I compared the 2H NMR spectra of B. subtilis with intact and compromised peptidoglycan layers.
- 2013 MSc, Universidad De Los Andes, Bogotá
Thesis title: Mechanical properties of membranes as a factor of resistance to Antimicrobial Peptides
Supervisor: Chad Leidy
- 2010, BSc, Universidad Nacional De Colombia
Research interests
- Biophysics
- Mechanics of Membrane
- NMR
- Antimicrobial Peptides
- NMR in whole cell
Publications
- Santisteban NP, Morrow MR, and Booth V. Effect of AMPs MSI-78 and BP100 on the lipid acyl chains of (2)H-labeled intact Gram positive bacteria. Biochim Biophys Acta Biomembr. 2020 May 1;1862(5):183199. DOI:10.1016/j.bbamem.2020.183199 |
- Booth V, Warschawski DE, Santisteban NP, Laadhari M, and Marcotte I. Recent progress on the application of (2)H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria. Biochim Biophys Acta Proteins Proteom. 2017 Nov;1865(11 Pt B):1500-1511. DOI:10.1016/j.bbapap.2017.07.018 |
- Santisteban NP, Morrow MR, and Booth V. Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR. Methods Mol Biol. 2017;1548:217-230. DOI:10.1007/978-1-4939-6737-7_15 |
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Events Participation
- Unraveling the Role of Peptidoglycan in the Interaction of Antimicrobial Peptides BP100 and MSI-78 with Bacterial Cell Envelopes through 2H NMR Experiments,Biophysical Journal, VOLUME 114, ISSUE 3, SUPPLEMENT 1, 454A, FEBRUARY 02, 2018. [1]
- 2H NMR Studies of Bacteria: How Does the Peptidoglycan Layer Modify the Interaction between Antimicrobial Peptides and the Lipid Membrane,Biophysical Journal, VOLUME 112, ISSUE 3, SUPPLEMENT 1, 380A-381A, FEBRUARY 03, 2017. [2]
- 2H NMR Studies of Living Bacteria Interacting with Antimicrobial Peptides,Biophysical Journal, VOLUME 110, ISSUE 3, SUPPLEMENT 1, 416A-417A, FEBRUARY 16, 2016. [3]
- The Antimicrobial Peptide Analog Magainin-H2 Induces Changes in the Phase Behavior and Lateral Organization of Supported Bilayers During Liquid-Crystalline/Gel Phase Coexistence,Biophysical Journal, VOLUME 102, ISSUE 3, SUPPLEMENT 1, 93A, JANUARY 31, 2012. [4]
