User:Paul Rothenberg/Notebook/Pauls Notebook/2013/07/15
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Reaction of OPD+H2O2 in Isopropanol Suspension with Mb/Tris Catalyst
We finished up the final trials of the reactions, and set the control buffers to lyopholize. They were flash frozen using the acetone-dry ice bath at -78°C.
Molar Absorptivity of 2,3 Diaminophenazine
We are now going to determine the molar absorptivity of 2,3 Diaminophenazine (DAPZ, to abbreviate it here).
We started by making a 1mM solution in H2</subO, using 21.023 mg of 2,3 Diaminophenazine. It did not all dissolve in solution, so we added that to a 250 mL volumetric flask and added an additional 150 mL of water, making sure to dissolve any remaining solid in the previous flask.
We then tried a 25 μM concentration by diluting the 50 μM solution with 1000 mL of water (dilution by a factor of 2). That was made, but we did not use that at all.
Dr. Hartings also suggested we dissolve the DAPZ in 1 mL of DMSO, and then add it to water. The concentration of this was 47,567 μM. Dissolving this in water, we got a 475.67 μM stock concentration.