User:Paul Rothenberg/Notebook/Pauls Notebook/2013/03/04

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4 March 2013 Objectives: 1. Synthesize two buffers to purify Nickel Hemoglobin previously synthesized Procedure: 1. 10 mM Tris (1.21g) and 50 mM NaCl (2.922g) were added, along with 900 mL of distilled water 2. The pH was brought to 9.5 using 1M NaOH and 3 M HCl, and the remaining 100 mL of distilled water were added 3. 10 mM Tris (1.21g) and 1M NaCl (58.44g) were added with 900 mL of distilled water 4. The pH was brought to 9.5 using 1M NaOH, and the remaining 100 mL of distilled water were added 5. 10 mM Tris and 50 mM NaCl were added along with 3,975 mL of distilled water 6. The pH was brought to 9.5 using 3M HCl, and the remaining 25 mL of water were added 7. The resulting buffer was used as a dialysis solution, exchanging previously synthesized Ni Hb in pH 8.5 solution 8. The Ni Hb was placed in two dialysis bags and placed in the pH 9.5 solution in a cold environment, left overnight