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BSA Fluorescence Solution
Stock Concentrations 160920
Volumes for making the solution:
This graph shows the Fluorescence intensity expelled from the pH 5 sample (0.25 mM Au, 3.125 BSA) over time (as it was heated by the machine). It is apparent that the fluorescence intensity increases in time, as the sample was heated.
This graph illustrated the emission maximums over time. The emissions increased until about 20 minutes into the experiment. After that the maximum emission was fluctuating just above 420 nm.
The graph above shows the integrated intensity of the sample over time. The curve appears to take a logarithmic patter. The Fluorescence increases quickly in the initial 20 minutes, reaching ~7400 nm. After that the Fluorescence continues to increase, but at a significantly slower rate, barely exceeding 9000 nm by the end of the experiment.
UV-Vis Sample Preparation for tomorrow
After the samples were prepared, they were left to cook in the thermooven at 80 degrees celcius for four hours.