User:Noy Kaufman/Notebook/EBC I AuNP/2017/09/12
Project name | Main project page Previous entry Next entry |
1. 250ml 0.1 M NaHCO3/Na2CO3 buffer solutions of pH 10, 11, & 12 were prepared by adding solid NaHCO3 to approximately 150ml of water. pH was measured and NaOH was added gradually while measuring pH until reading to desired value. Table 1.buffer pH adjustment volumes
3. 10 mM HAuCl4 solution and a 100 mM arginine solution were prepared. AuNP solutions were prepared using 10:1, 1:1, & 1:10 Au:AA:buffer concentration ratio (1:0.01:8.99, 1:0.1:8.9, 1:1:8 volumetric ratios)for each pH (10,11,12). The 18 AuNP solutions were left for overnight heating. 4. Buffer for Bradford Analysis was prepared using 1L 50mM Tris 50mM NaCl pH 7.5 5. Buffer for Dialysis was prepared using 100 mL Glycine Buffer with pH = 3.5 Table 2. glycine buffer pH scaling
NK |