User:Noy Kaufman/Notebook/EBC I AuNP/2017/08/29

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8/29/17 '''Making Stock solutions''' 1. Prepared 50ml of 2.5mM HAuCl4 solution: -=2.5*10^-3 M 339.70g/mol *2.5*10^-3 mol/L*0.05L = 0.04247 g of solid add to 50ml volumetric flask and DI water added to reach a total of 50 ml. 2. prepared 50ml of 250µM BSA solution: -= 2.5*10^-4 M 65,000g/mol* 2.5*10^-4 nol/L*0.05L= 0.8125 g of solid add to 50ml volumetric flask and DI water added to reach a total of 50 ml. '''Making AuNP''' total of 10ml using 1ml of HAuCl4* 1. Au:BSA = 80:1 - 1ml Au added to 0.125ml BSA and 8.825ml H2O in a glass test tube. 2. Au:BSA = 160:1 - 1ml Au added to 0.0625ml BSA and 8.9375ml H2O in a glass test tube. the test tubes were labeled, covered with aluminum foil and placed in the oven at 80C for 3-4 hours. '''Testing the accuracy of pipettes''' room tmp was determined to be 27.8C. density of water at 27.8C was found to be 0.99658 g/cm^3 using P-200 water was added to a glass vial in 200µL increments. The weight of the sample was measured after each addition and using the density value and excel the actual value was determined for 6 measurements (see table 1). Table 1: results for pipette accuracy test It can be seen that the % error were around 0.9-1.5% and that the second measurement showed the highest change between theoretical value to observed value. '''Preparing solutions''' 1. 100 mL of 1.71 mM (=1.71*10^-3M) NaCl from solid: 1 moleNaCl= 58.44g --> 1.71*10^-3mole/L*58.44g/mole*0.1L = 0.00990324 g od solid in a 100ml volumetric flask and water added to fill up to line. 2. 25 mL of 13.5 μM (=13.5*10^-6M) dye from stock (161*10^-3M) & wet flask: 13.5*10^-6M*0.025L= 161*10^-3M*V2 V2= 0.002096ml of stock dye added to 25ml volumetric flak and water added to fill up to line. '''Reagent Purities''' 1. tested tap, DI, and HPLC water using conductivity Table 2: purity results Water type conductivity (µS/cm) DI 1.94 HPLC (open). 2.24 Tap 3388 it can be seen that tap water is the least pure form tested. In theory, HPLC water is the purest form, however, the tested HPLC water bottle was left open and was therefore subject to air contamination (CO2) 2. Cleanliness od glasses tested by filling it with water and placing in a UV VIS machine and then in a fluorescence detector figure 1: UV VIS results peak can be seen at around 372nm, therefore indicating that the glass is not clean. Figure 2: fluorescence results a large peak can be seen 440nm NK