User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/04/08

Biomaterials Design Lab

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Objectives Centrifuge 2nd procedure Ag:BSA NPs UVvis of Ag:BSA NPs from last time Put dry-autoclaved scaffolds into bacteria Make Au nanofibers and print scaffolds (12) for brute force Sample Prep: Nanofibers for Brute Force Solutions: Au: 0.011g in 10mL to make 2.793mM solution Myoglobin: 0.011g in 10mL to make 0.0621mM solution Brute Force Nanofiber-coated Scaffolds 4 PLA scaffolds printed for each test tube Gold:Myoglobin (100:1) nanofibers synthesized by above method Nanofibers dried onto PLA scaffolds Scaffolds submerged in water Autoclaved Results: UV-vis Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm Sample Prep: Scaffolds in Bacteria E. coli bacteria culture were prepared Bacteria culture media was prepared and placed in 5 small test tubes, each tube for each type of scaffold treatment E. coli were placed in test tubes 3 Dry-autoclaved scaffolds submerged in each test tube of bacteria solution Test tubes with cells and scaffolds were placed in shaker until next class