User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/08/27
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August 27, 2014
Preparation of Stock Solutions & Culture Tubes
We made 50 mL of a 2.512 mM solution of HAuCl4.
We then made 50 mL of a 250 μM solution of lysozyme.
Finally, we made 50 mL of a 2.512 x 10-4 M solution of HCl.
NOTE: this needs to be re-done to match the concentration of the HAuCl4 stock, or 2.512 mM.
6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.
NOTE: The amount of Au in each tube was intended to be 0.5 mL, or 1/10 the volume of the final solution. Using a stock solution with a [H+] of 2.512 mM, the concentration of [H+] in the final 5 mL solution would be 2.512 x 10-4. This would give a solution of 3.6. Because we used 2.5 mL of Au in each tube, or 1/2 the volume of the final solution, our culture tubes contained a higher [H+] and therefore had a lower pH of 2.9.
Each test tube was covered in foil and placed in an oven at 80°C for 3 hours.
Kinetics of AuNP Formation