User:Michael S. Bible/Notebook/571/2014/11/11

Biomaterials Design Lab

Main project page Previous entry

Objective Analyze Dialysis Started on 11/5 UV-Vis of Protein solutions KI ISE of Ligand solutions Fluorescence Analysis of Protein Solutions Bradford Analysis of Protein Solutions Bradford Analysis Bradford was performed using: 1:4 Bio-Rad Solution 50 μL of protein solutions 200 μL of Bradford Reagent 750 μL of 50 mM NaCl/Tris Buffer UV Analysis of KI Dialyzed Lysozyme KI ISE Data Original KI Concentration (mM) mV Measurement (mV) Final KI Concentration (mM) 66.65 -253.4 16.79 39.41 -243.8 11.21 19.69 -239.3 9.28 11.45 -220.6 4.23 3.23 -193.5 1.35 Fluorescence Fluorescence was run from λ = 300 - 550 nm and at an excitation wavelength of λ=280 nm. New Dialysis Experiment A new dialysis was set up using: 0.6 g/L 50 mM, 5 mM, 500 μM, 50 μM, 5 μM CaCl2