User:Michael S. Bible/Notebook/571/2014/10/29

Biomaterials Design Lab

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Objective Create new CaCl2 solutions to dialyze against. Collect data from dialysis started yesterday. Start a new dialysis of Lysozyme against CaCl2. Data Ca2+ ISE for Lysozyme dialzyed against CaCl2 Original CaCl2 Concentration (mM) mV Measurement (mV) Final CaCl2 Concentration (mM) 50 78.5 22.57 5 52.8 2.20072 0.5 20.8 0.1213 0.05 1.8 0.0217 0.005 -0.3 0.0179 (This is a bad data point) Notes The Bradford analysis of this sample turned out poorly again and did not yield workable results. New CaCl2 solutions have the following concentrations: 6.83 mM 1.87 mM 0.68 mM 0.020 mM 0.00058 mM